Highly sensitive microplate ß-galactosidase assay for yeast two-hybrid systems

Brouchon-Macari, L., Joseph, M., Dagher, M. Highly sensitive microplate ß-galactosidase assay for yeast two-hybrid systems. BioTechniques Vol. 35, No. 3, 446-448 (2003).

“Culture for 20 h with shaking in a deep-well, conical-bottom microplate.” (Yeast Culture protocol)

“Shake at 800 rpm for 10 min at 30°C.” (ß-Galactosidase Assay)

Localized transfection on arrays of magnetic beads coated with PCR products

salan, M., Santori, M., Gonzalez, C., Serrano, L. Localized transfection on arrays of magnetic beads coated with PCR products. Nature MethodsVol. 2, No. 2,113-118 (2005).

“We pipetted 10 µl bead aliquots in a 96-well plate, adding 5 µl of water plus 5 µl of PCR product per well. After mixtures were incubated for 3 h at 22 °C, with mixing at 800 r.p.m, ...”

“We incubated the plate for 45 min at 22 °C with mixing at 800 r.p.m ...”

Microplate Nitrate Test Kit — Standard Range

Microplate Nitrate Test Kit — Standard Range, The Nitrate Elimination Co., Inc., Lake Linden, Michigan.

“Pipet 90 µl NaR-Assay Buffer-NADH solution (prepared in Step 8 of Reagent Preparation) to each well. Shake on a plate mixer for ~20 minutes at 800 rpm.”

“Add 50 µl Color Reagent No. 1 and 50 µl Color Reagent No. 2 solution to each well. Shake on a plate mixer for ~10 minutes at 800 rpm.”

Scalable high-throughput micro-expression device for recombinant proteins

Page, R. et al. Scalable high-throughput micro-expression device for recombinant proteins. BioTechniques Vol. 37, No. 3, 364-370 (2004).

“[sterile 2-mL, 96-deep-well, round-bottom blocks are] grown at 900 rpm and 37°C.”

“...cultures are allowed to shake at 900 rpm and 37°C for an additional 4-5 h.”